NIH approves 27 more stem cell lines

By Dan Vergano,

National Institutes of Health chief Francis Collins approved another 27 human embryonic stem cell lines for federal research funding Monday, but limited support to diabetes-related pancreatic cell experiments.

The 27 Harvard University cell lines join 13 from Children’s Hospital Boston added earlier this month to a NIH stem cell registry. Human embryonic stem cells are the precursors to every type of tissue. Cell “lines”, or colonies, are grown from the collected inner cells of an embryo destroyed in the process of collection. Researchers hope to study organ development, screen drugs and someday perhaps grow rejection-free transplant tissues from the cells.

In an Oct. 12 application for NIH approval, the Harvard researchers had noted they gathered the embryos from donations by fertility clinic patients, using consent forms that listed their use as diabetes research. For that reason, “NIH-funded research with this line is limited,” says the registry, to “study the embryonic development of (tissue) with a focus on pancreatic formation,” with the aim of “producing cells that produce insulin, for transplantation into diabetics.” An advisory committee last week had recommended the limitation, and Collins apparently agreed with their recommendation.

The Harvard cells have been used in privately-funded studies of ALS, Alzheimer’s disease, Huntington’s disease, Parkinson’s disease, spinal injury, heart disease, cancer infertility and other ailments. A Proceedings of the National Academies of Science journal report last year called them the “gold standard”  for comparison with cells claimed to possess tissue-forming properties.

The approval puts 40 cell lines on the NIH registry, beating the Bush Administration 2001 to 2008 total of 21 lines on its registry.

Scientists use Dental Stem Cells in First Successful Human Transplant


BioEDEN, the world-leader in dental stem cell extraction and storage, is celebrating the first successful human medical trial using stem cells from teeth.

Scientists at The Second University of Naples, Italy have successfully used stem cells taken from dental pulp found in teeth to create new bone tissue and graft it onto a human jaw. The success yields a vast number of medical possibilities for dental stem cells, and for those people who store them for future use.

BioEDEN, based in Daresbury, Cheshire, with laboratory facilities in Austin Texas, and Bangkok Thailand, holds the global patent for the extraction, cryopreservation and storage of dental stem cells for medical use. BioEDEN stores stem cells found in children’s milk teeth, with the hope that one day they will save the donor’s life by being able to repair damaged or diseased tissue.

“For BioEDEN, this achievement is an immaculate demonstration of how vital dental stem cells will come to be in the future of medicine,” said Jim Curtis, Managing Director of BioEDEN. “This is the first time dental stem cell research has moved from the laboratory to human medical trials, and the announcement is truly groundbreaking. It opens up a great deal of medical hope for the future.”

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New human embryonic stem cell lines eligible for federal research dollars for the first time since 2001

By Karen Kaplan,

The number of human embryonic stem cell lines eligible to be used in government-funded research just went up by 13.

The National Institutes of Health announced today that 11 new cell lines from Dr. George Daley at Children’s Hospital Boston and two lines from Ali Brivanlou at Rockefeller University in New York became the first additions to the NIH Human Embryonic Stem Cell Registry since President Obama reversed his predecessor’s policy. Under President Bush, only human embryonic stem cells prior to August 2001 were eligible for federal funding.

The new lines were derived from embryos created for fertility treatments and donated by couples who went through a rigorous informed consent process.

And more may be on the way. The NIH said that 96 more lines have been submitted by researchers, including 20 that will be vetted by an advisory committee on Friday.

The additions come nearly nine months after Obama signed an executive order that directed the NIH to make federal research funds available to newer lines of human embryonic stem cells. Scientists were overjoyed and said the decision would accelerate the pace of research into such ailments as diabetes, Alzheimer’s and spinal cord injuries. Details of the policy are available here.

Stem Cells’ Next Use: Fighting Extinction


It’s a lonely world for the two northern white rhinos at Escondido’s Wild Animal Park. They are among less than a dozen of their kind left on Earth.

Conservationists work constantly through habitat protection and other means to save these and other endangered species. And now they are adding a new technology to their list of possible solutions to extinction — stem cells.

Scientists at the San Diego Zoo’s Institute for Conservation are working on two separate projects that employ some of the same stem cell breakthroughs that might someday treat disorders like Alzheimer’s and Parkinson’s diseases in humans.

“This is a very preliminary experiment,” said Oliver Ryder, director of genetics at the Institute for Conservation Research. “We want to see if the process that’s worked on human cells will work in animals.”

Ryder’s group wants to reprogram adult cells from drill monkeys and northern white rhinos into stem cells. Using a type of virus called a retrovirus, scientists introduce genes into the DNA of an adult cell that cause it to behave like an embryonic stem cell, a versatile cell that can divide to form any other cell type in the body.

The Zoo researchers are working in collaboration with world-renowned stem cell researcher Jeanne Loring and her lab at the Scripps Research Institute.

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First Reconstitution of an Epidermis from Human Embryonic Stem Cells


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Stem cells & treating tumors


Human embryonic stem cells could help people with learning and memory deficits after radiation treatment for brain tumors, suggests a new UC Irvine study.

Research with rats found that transplanted stem cells restored learning and memory to normal levels four months after radiotherapy. In contrast, irradiated rats that didn’t receive stem cells experienced a more than 50 percent drop in cognitive function.

“Our findings provide the first evidence that such cells can be used to ameliorate radiation-induced damage of healthy tissue in the brain,” says Charles Limoli, UCI radiation oncology associate professor and senior author of the study, appearing online the week of Nov. 9 in the Proceedings of the National Academy of Sciences.

Stem Cells: Scientists Successfully Reprogram Blood Cells


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Scientists Map First Complete Human Epigenome, The Driver Of Gene Expression


When scientists mapped the DNA sequence of 3 billion bases in the human genome they uncovered the master blueprint of what makes a human being; now a team in the US has produced a high resolution map of the first complete human epigenome, the driver of gene expression that regulates how all the options offered in the genome are put together to make the unique person that grows in a particular environment. Understanding the epigenome is the key to understanding how genes affect health and disease under the influence of factors like lifestyle, diet and environment.

The work is described in a paper published in the 14 October online issue of Nature.

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Surgeons ‘Grow’ New Cheekbones for 15-Year-Old Born With Rare Condition

A 15-year-old boy born without cheekbones has a new lease on life after surgeons at Cincinnati Children’s Hospital used a first-of-its-kind procedure to reconstruct the teen’s face.

They did it by using a combination of donor bone, growth hormone and the teenager’s own stem cells, The Cincinnati Enquirer reported.

Brad Guilkey was born with Treacher Collins Syndrome, which is a rare genetic condition that affects the development of bones and other tissues in the face. It’s passed down through families and is estimated to occur in 1 in 50,000 people, according to the National Institutes of Health.

Brad was born without zygomatic bones, which is the arch of bone beneath the eye that forms the prominence of the cheek. Because of the lack of this bone, the teenager was left with a “sunken-cheek” look.

In order to fix this, Dr. Jesse Taylor, the surgeon in the division of craniofacial and pediatric plastic surgery, first carved a model of the missing bones from cadaver bone.

Next, Taylor and his team injected the cadaver bone with stem cells harvested from Brad’s stomach fat and a type of growth hormone, called Bone Morphogenic Protein-2, which signals stem cells to turn into bone cells.

The team then wrapped the whole construct in a piece of periosteum — the thick membrane covering the entire surface of a bone – which was harvested from Brad’s thigh. Finally, they placed the bone constructs in Brad’s skull.

A few months after the surgery, doctors received the news they were hoping for: CT scans showed new living bone had grown in place in Brad’s skull.

Taylor said this technique gives doctors a new option for treating children and adults who have lost bone to disease or traumatic injury.

Jaw bone created from stem cells


Scientists have created part of the jaw joint in the lab using human adult stem cells.

They say it is the first time a complex, anatomically-sized bone has been accurately created in this way.

It is hoped the technique could be used not only to treat disorders of the specific joint, but more widely to correct problems with other bones too.

The Columbia University study appears in Proceedings of the National Academy of Sciences.

The bone which has been created in the lab is known as the temporomandibular joint (TMJ).

Problems with the joint can be the result of birth defects, arthritis or injury.

Although they are widespread, treatment can be difficult.

The joint has a complex structure which makes it difficult to repair by using grafts from bones elsewhere in the body.

The latest study used human stem cells taken from bone marrow.

These were seeded into a tissue scaffold, formed into the precise shape of the human jaw bone by using digital images from a patient.

The cells were then cultured using a specially-designed bioreactor which was able to infuse the growing tissue with exactly the level of nutrients found during natural bone development.

Big potential
Lead researcher Dr Gordana Vunjak-Novakovic said: “The availability of personalised bone grafts engineered from the patient’s own stem cells would revolutionise the way we currently treat these defects.”

Dr Vunjak-Novakovic said the new technique could also be applied to other bones in the head and neck, including skull bones and cheek bones, which are similarly difficult to graft.

The option to engineer anatomically pieces of human bone in this way could potentially transform the ability to carry out reconstruction work, for instance following serious injury or cancer treatment.

She said: “We thought the jawbone would be the most rigorous test of our technique; if you can make this, you can make any shape.”

She stressed that the joint created in the lab was bone only, and did not include other tissue, such as cartilage. However, the Columbia team is working on a new method for engineering hybrid grafts including bone and cartilage.

Another major challenge for scientists will be to find a way to engineer bone with a blood supply that can be easily connected to the blood supply of the host.

Professor Anthony Hollander, a tissue engineering expert from the University of Bristol who helped produce an artificial windpipe last year, said there was still a lot of work to be done before the new bone could be used on patients.

But he said: “One of the major problems facing scientists in this field is how to engineer a piece of bone with the right dimensions – that is critical for some of these bone defects.

“This is a lovely piece of tissue engineering which has produced bone with a high degree of accuracy in terms of shape.”